Figure 1: FACS analysis of cell surface expression of mouse CTLA4 on CHO-K1/mouse CTLA4 cells. The CHO-K1/mouse CTLA4 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE anti-mouse CD152 (Cat. No. 106305, Biolegend).

CHO-K1/mouse CTLA4 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated protein 4 (CTLA4) on the surface. The surface expression of mouse CTLA4 is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse CTLA4 and for evaluating target binding affinity.

產品編號	M00570
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress Mus musculus cytotoxic T-lymphocyte-associated protein 4 (CTLA4) on the surface. The surface expression of mouse CTLA4 is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse CTLA4 and for evaluating target binding affinity.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Freeze Medium-DATA	95% Culture Medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1: FACS analysis of cell surface expression of mouse CTLA4 on CHO-K1/mouse CTLA4 cells. The CHO-K1/mouse CTLA4 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE anti-mouse CD152 (Cat. No. 106305, Biolegend).

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CHO-K1/mouse CTLA4 Stable Cell Line

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