| Product Description |
Recombinant CHO-K1 cells stably overexpress human TNF receptor superfamily member 9 (4-1BB) on the surface. The surface expression of 4-1BB is validated by FACS analysis. |
| Culture Properties |
Adherent |
| Stability |
Stability: Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size |
Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA |
95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
Figure 1. FACS analysis of cell surface expression of 4-1BB on CHO-K1/4-1BB cells. The CHO-K1/4-1BB cells (Blue) and the negative control CHO-K1 cells (Red) were probed using 4-1BB Antibody (Cat. No. A01974, Genscript) and PE conjugated Goat anti Mouse IgG (H+L) (Cat. No. ab6785, Abcam).
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
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