Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).

CHO-K1/VISTA Stable Cell Line

Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.

產(chǎn)品編號(hào)	M00533
規(guī)格	2 vials 大單詢(xún)價(jià)
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價(jià)格	詢(xún)價(jià)


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Product Description	Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Freeze Medium-DATA	95% Complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)

圖例

Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).

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CHO-K1/VISTA Stable Cell Line

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