Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC₅₀ of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.

CHO-K1/Gα15/CXCR5

Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.

產品編號	M00526
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. BCA-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CXCR5 agonist, BCA-1 (Cat No.: Z02826, GenScript). The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of BCA-1 (mean ± SEM, n = 3). The EC₅₀ of BCA-1 on CHO-K1/Gα15/CXCR5 cells was 0.11 μM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/Gα15/CXCR5

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