Figure IL-8 induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CXCR1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist IL-8. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of IL-8 (Mean ± SD, n = 2). The EC50 of IL-8 on this cell was 0.11 μg/ml.
CHO-K1/Gα15/CXCR1
Chemokine (C-X-C motif) receptor 1 (CXCR1) is a rhodopsin-like G protein-coupled receptor. It is one of two high-affinity receptors for the CXC chemokine interleukin-8 (IL-8), a major mediator of immune and inflammatory responses. The structure of human CXCR1 in a lipid bilayer should help to facilitate the discovery of new compounds that interact with GPCRs. GenScript’s human CXCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay.
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