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目錄產(chǎn)品 » 穩(wěn)定細(xì)胞系 » Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line

Figure Dose dependent stimulation of intracellular calcium mobilization upon treatment with its ligand MIP-1α. The CCR5-expressing stable subline was loaded with Calcium-4 prior to being stimulated with a CCR5 receptor agonist, human MIP-1α (GenScript, Cat No: Z03137). The intracellular calcium mobilization was monitored by FLIPR. P1, P5, P10, and P20 represent the cell passage number.

CHO-K1/Gα15/CCR5

C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay.

產(chǎn)品編號	M00523
規(guī)格	2 vials 大單詢價
數(shù)量
價格	詢價


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Synonyms

CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

產(chǎn)品簡介

Storage

Liquid nitrogen immediately upon receiving

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS, 3 μg/ml puromycin (Cat. #A11138-03, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)