Figure 1. Angiotensin II-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/AT1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist angiotensin II. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of angiotensin II (Mean ± SEM, n = 3). The EC₅₀ of angiotensin II on this cell was 1.23 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with angiotensin II in HEK293/AT1 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in HEK293/AT1 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of angiotension II on HEK293/AT1 cells was 3.53 nM.

HEK293/AT1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human angiotensin II receptor type 1 (AT1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.

產品編號	M00458
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant HEK293 cells stably overexpress human angiotensin II receptor type 1 (AT1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	DMEM, 10% FBS, 3 μg/ml puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	DMEM, 10% FBS
Freeze Medium-DATA	50% DMEM (Cat. No. 10566, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 5% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. Angiotensin II-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/AT1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist angiotensin II. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of angiotensin II (Mean ± SEM, n = 3). The EC₅₀ of angiotensin II on this cell was 1.23 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with angiotensin II in HEK293/AT1 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in HEK293/AT1 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of angiotension II on HEK293/AT1 cells was 3.53 nM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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HEK293/AT1 Stable Cell Line

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