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目錄產品 » 穩定細胞系 » Human Recombinant ADRA2B Adrenoceptors Stable Cell Line

Figure UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2B/Gqi5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of UK14304 (Mean ± SD, n = 2). The EC₅₀ of UK14304 on this cell was 0.12 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/ADRA2B/Gqi5 Stable Cell Line

Adrenoceptors are 7-transmembrane receptors which mediate the central and peripheral actions of the neurotransmitter, noradrenaline (norepinephrine), and the hormone and neurotransmitter, adrenaline (epinephrine). Based on both pharmacological and molecular evidence, they are divided into three major types - α1, α2, and β. The α2 adrenoceptors include 3 subtypes - α2A, α2B, and α2C - each of which couples to Gi/o protein primarily. The α2B adrenoceptors (ADRA2B) is expressed in the kidney, liver, brain, lung, heart, and skeletal muscle. The development of α2B and α2C knock-out mice has shown that these two subtypes are not involved in the central hypotensive response to α2 agonists.

產品編號	M00454
規格	2 vials 大單詢價
數量
價格	詢價


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Synonyms

Alpha-2B adrenoceptor, Adrenergic alpha2B- receptor class III, adrenergic receptor, alpha-2B adrenergic receptor, alpha-2B adrenoceptor, alpha-2BAR, alpha2B, [a]2B, Adra-2b

產品簡介

Storage

Liquid nitrogen immediately upon delivery

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)