Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a DP1 receptor agonist, PGD2. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC₅₀ of PGD2 on this cell was 18.5 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/DP1/Gα15 Stable Cell Line

DP1 is a Gs-coupled GPCR expressed in the colon, eye, platelets, eosinophils, that bind to PGD2 and PGE2. DP1 can inhibit platelet aggregation, and also inhibit leukotriene B4 and superoxide anion (O2-) release from human neutrophils. It can relax smooth muscle and regulate eosinophil apoptosis. DP receptor knockout OVA-challenged mice exhibit reduced Th2 cytokine levels and lymphocyte accumulation in the lung. In addition, they failed to produce asthmatic responses.

產(chǎn)品編號(hào)	M00453
規(guī)格	2 vials 大單詢價(jià)
數(shù)量
價(jià)格	詢價(jià)


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Synonyms

Human Recombinant Prostanoid Receptor DP1 Stable Cell Line

產(chǎn)品簡(jiǎn)介

Storage

Liquid nitrogen immediately upon delivery

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)

圖例

Figure 1. PGD2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/DP1/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a DP1 receptor agonist, PGD2. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PGD2 (Mean ± SD, n = 2). The EC₅₀ of PGD2 on this cell was 18.5 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

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CHO-K1/DP1/Gα15 Stable Cell Line

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