Figure 1. Orphanin FQ-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with Orphanin FQ. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Orphanin FQ (Mean ± SEM, n = 3). The EC₅₀ of Orphanin FQ on this cell was 236.1 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/OPRL1/Gα15 Stable Cell Line

Following the cloning of the classical opioid receptors (mu, delta and kappa), the opiate receptor like-1 (ORL1) was identified as a G-protein coupled receptor (GPCR) with 65% structure homology to the other members of the opioid family. OPRL1 is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ and may be involved in the regulation of numerous brain activities, particularly instinctive and emotional behaviors. Recently, new study results are consistent with the reported high density of ORL1 receptor mRNA in dorsal raphe nucleus and with inhibitory actions of nociceptin in cells expressing ORL1.

產(chǎn)品編號	M00447
規(guī)格	2 vials 大單詢價
數(shù)量
價格	詢價


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Synonyms

Human Recombinant Opiate Receptor-like 1 Stable Cell Line

產(chǎn)品簡介

Storage

Liquid nitrogen immediately upon delivery

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)

圖例

Figure 1. Orphanin FQ-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OPRL1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with Orphanin FQ. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Orphanin FQ (Mean ± SEM, n = 3). The EC₅₀ of Orphanin FQ on this cell was 236.1 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

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CHO-K1/OPRL1/Gα15 Stable Cell Line

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