Figure 1. CaCl₂-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CASR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist CaCl₂. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of CaCl₂ (Mean ± SD, n = 2). The EC₅₀ of CaCl₂ on this cell was 0.43 mM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/CASR Stable Cell Line

CASR is a calcium-sensing receptor and plays an important role in regulating PTH secretion. It is expressed in many different tissues, such as parathyroid, pancreatic islet and insulinoma cells, breast, gastrinoma cells, pituitary, keratinocytes and human colon epithelial cells. CASR Inhibits PTH secretion and parathyroid cellular proliferation in parathyroid glands or cells, and also inhibits proliferation of colonic crypt cells. Besides, it can stimulate PTHrP secretion in prostate cell line, and stimulate calcitonin secretion from thyroidal C-cells.

產品編號	M00434
規格	2 vials 大單詢價
數量
價格	詢價


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Synonyms

Human Recombinant Calcium Sensor Receptor Stable Cell Line

產品簡介

Storage

Liquid nitrogen immediately upon delivery

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)

圖例

Figure 1. CaCl₂-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CASR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist CaCl₂. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of CaCl₂ (Mean ± SD, n = 2). The EC₅₀ of CaCl₂ on this cell was 0.43 mM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

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CHO-K1/CASR Stable Cell Line

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