Figure 1. Buserelin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GNRHR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist buserelin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of buserelin (Mean ± SD, n = 2). The EC₅₀ of buserelin on this cell was 20.2 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/GNRHR/Gα15 Stable Cell Line

The gonadotropin-releasing hormone receptor (GNRHR), also known as the luteinizing hormone releasing hormone receptor (LHRHR), is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Upon activation, the LHRHr stimulates tyrosine phosphatase and elicits the release of LH from the pituitary. Evidence exists showing the presence of LHRH and its receptor in extrapituitary tissues as well as a role in progression of some cancers.

產品編號	M00426
規格	2 vials 大單詢價
數量
價格	詢價


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Synonyms

GnRH receptor, luteinizing hormone-releasing hormone (LHRH) receptor, LHRHR, LRHR, GRHR, GNRHR1, luliberin receptor, GnRH1, Type I GnRHR, LHRH receptor, GnRH I receptor, GH1, gnRH receptor, gnRH-R, gonadotropin-releasing hormone receptor, growth hormone

產品簡介

Storage

Liquid nitrogen immediately upon delivery

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)

圖例

Figure 1. Buserelin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GNRHR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist buserelin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of buserelin (Mean ± SD, n = 2). The EC₅₀ of buserelin on this cell was 20.2 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

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CHO-K1/GNRHR/Gα15 Stable Cell Line

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