Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTGER2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SD, n = 2). The EC₅₀ of PGE2 on this cell was 0.25 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/EP2/Gα15 Stable Cell Line

Prostaglandin (PG) E2 exerts its actions by acting on a group of G-protein-coupled receptors (GPCRs). There are four GPCRs responding to PGE2 designated subtypes EP1, EP2, EP3, and EP4 and multiple splicing isoforms of the subtype EP3. The EP subtypes exhibit differences in signal transduction, tissue localization, and expression regulation. Studies have identified that EP2 mediates many processes, such as facilitating ovulation and fertilization, suppressing dendritic cell differentiation, and promoting amyloid-β formation in Alzheimer's disease.

產品編號	M00311
規格	2 vials 大單詢價
數量
價格	詢價


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Synonyms

prostaglandin E, prostaglandin E receptor 2, 53kD, Ptger-ep2, PGE receptor EP2 subtype, prostaglandin E receptor 2, prostaglandin E receptor EP2 subtype, prostaglandin E2 receptor EP2 subtype, prostaglandin E2 receptor EP2 subtype-like, prostaglandin E2 receptor type 2, prostanoid EP2 receptor, Ptgerep2

產品簡介

Storage

Liquid nitrogen immediately upon delivery

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. #10131-035, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)

圖例

Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTGER2/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SD, n = 2). The EC₅₀ of PGE2 on this cell was 0.25 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

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CHO-K1/EP2/Gα15 Stable Cell Line

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