Figure 1. UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of UK14304 (Mean ± SEM, n = 3). The EC₅₀ of UK14304 on this cell was 2.68 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/ADRA2A/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 2A (ADRA2A) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.

產品編號	M00281
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 2A (ADRA2A) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 200 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2A/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of UK14304 (Mean ± SEM, n = 3). The EC₅₀ of UK14304 on this cell was 2.68 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/ADRA2A/Gα15 Stable Cell Line

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