Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA1B cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Epinephrine. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Epinephrine (Mean ± SEM, n = 3). The EC₅₀ of Epinephrine on this cell was 17.55 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with epinephrine in CHO-K1/ADRA1B cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in CHO-K1/ADRA1B cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of epinephrine on CHO-K1/ADRA1B cells was 0.11 μM.

CHO-K1/ADRA1B Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 1B (ADRA1B) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.

產品編號	M00260
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 1B (ADRA1B) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. Epinephrine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA1B cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Epinephrine. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Epinephrine (Mean ± SEM, n = 3). The EC₅₀ of Epinephrine on this cell was 17.55 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with epinephrine in CHO-K1/ADRA1B cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in CHO-K1/ADRA1B cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of epinephrine on CHO-K1/ADRA1B cells was 0.11 μM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/ADRA1B Stable Cell Line

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