Figure 1. Oxotremorine M-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist oxotremorin M. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of oxotremorin M (Mean ± SEM, n = 3). The EC₅₀ of oxotremorin M on this cell was 41.4 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with oxotremorin M in CHO-K1/M4/Gα15 cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/M4/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of oxotremorin M on CHO-K1/M4/Gα15 cells was 14.45 nM.

Figure 3. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with oxotremorin M in CHO-K1/M4/Gα15 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in CHO-K1/M4/Gα15 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of oxotremorin M on CHO-K1/M4/Gα15 cells was 0.89 μM.

CHO-K1/M4/Gα15 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 4 (M4) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.

產品編號	M00238
規格	2 vials 大單詢價
數量
價格	詢價


聯系我們

產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human cholinergic receptor muscarinic 4 (M4) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. Oxotremorine M-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist oxotremorin M. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of oxotremorin M (Mean ± SEM, n = 3). The EC₅₀ of oxotremorin M on this cell was 41.4 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with oxotremorin M in CHO-K1/M4/Gα15 cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/M4/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of oxotremorin M on CHO-K1/M4/Gα15 cells was 14.45 nM.

Figure 3. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with oxotremorin M in CHO-K1/M4/Gα15 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in CHO-K1/M4/Gα15 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of oxotremorin M on CHO-K1/M4/Gα15 cells was 0.89 μM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

CHO-K1/M4/Gα15 Stable Cell Line

資源中心

技術支持

關于我們