Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/EP1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SEM, n = 3). The EC₅₀ of PGE2 on this cell was 0.134 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

HEK293/EP1 Stable Cell Line

Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.

產(chǎn)品編號(hào)	M00228
規(guī)格	2 vials 大單詢(xún)價(jià)
數(shù)量
價(jià)格	詢(xún)價(jià)


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產(chǎn)品簡(jiǎn)介

Product Description	Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養(yǎng)條件

Culture Medium	DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 250 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium	DMEM, 10% FBS
Freeze Medium-DATA	50% DMEM (Cat. No. 10566, Gibco), 45% FBS (Cat. No. 10099-141, Gibco), 5% DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/EP1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PGE2. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PGE2 (Mean ± SEM, n = 3). The EC₅₀ of PGE2 on this cell was 0.134 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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HEK293/EP1 Stable Cell Line

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