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目錄產品 » Cas9 ELISA Kit

Cas9 ELISA Kit

Cas9 ELISA Kit provides a rapid and quantitative solution for detecting Cas9 protein in cell lysates, viral vectors, and gene-editing reagents.
¥5480
L01069

Linearity Range 0.125 to 8 ng/mL
Sensitivity LLOQ:0.125ng/ml
Precision Intra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%
Accuracy 80%-120%
Standard 3.2 μg/mL of Cas9 protein
Suitable Sample Type cell lysates, viral vectors, and gene-editing reagents
Specificity This kit is designed for the detection of a wide range of Cas9 proteins, including wild-type SpCas9, high-fidelity mutants (e.g., eSpCas9), and variants featuring nuclear localization signals (NLS) at the N-terminus, C-terminus, or both.
Assay Temperature 37°C
Assay Time 3 h
Storage The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
Kit Contents
Component Quantity/Size Part No.
Cas9 Capture Plate 1 plate B2-80
Cas9 Standard Stock 1 vial (50 μL) B2-10
Biotin Anti-Cas9 Antibody 1 bottle (12 mL) B2-20
Streptavidin-HRP 1 bottle (12 mL) B2-30
Sample Dilution Buffer 1 bottle (60 mL) B2-60
20× Wash Solution 1 bottle (60 mL) B-A1-71
TMB Solution 1 bottle (12 mL) B-A1-40
Stop Solution 1 bottle (6 mL) B-A1-50
Plate Sealer 2 pieces /
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Cas9 ELISA Kit

Figure 1. Typical Cas9 standard curve fitted with a four-parameter logistic model. ?

Cas9 ELISA Kit

Figure 2: Cas9 variants curves ?

Cas9 ELISA Kit

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Cas9 ELISA Kit

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Cas9 ELISA Kit

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Cas9 ELISA Kit

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Cas9 ELISA Kit

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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