国产精品久久久久久永久牛牛,55国产福利在线视频,成人午夜精品视频在线观看

目錄產品 » GenCRISPR™/Cas9 基因編輯相關產品 » Cas9核酸酶 » GenCRISPR? Cas12a (Cpf1) Nuclease
GenCRISPR? Cas12a (Cpf1) Nuclease

A 20 μl reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng GenCRISPR? Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR? Cas12a (Cpf1) Nuclease

The clustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonly present in archaea and bacteria. The CRISPR systems enable the host to specifically target and cleave foreign nucleic acids thus targeting infectiousviruses and plasmids. Recently, a type V CRISPR system has been identified in several bacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast to Cas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require an additional trans-activating crRNA (tracrRNA), and allow for targeting of additional genomic regions by cleaveing the target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requires a G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. Recombinant Acidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli and purified. The nuclease contains nuclear localization sequence (NLS) at the C-terminus and 6× His-tag at the C-terminus.
Z03502
¥915

聯系我們
Description The clustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonly present in archaea and bacteria. The CRISPR systems enable the host to specifically target and cleave foreign nucleic acids thus targeting infectiousviruses and plasmids. Recently, a type V CRISPR system has been identified in several bacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast to Cas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require an additional trans-activating crRNA (tracrRNA), and allow for targeting of additional genomic regions by cleaveing the target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requires a G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. Recombinant Acidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli and purified. The nuclease contains nuclear localization sequence (NLS) at the C-terminus and 6× His-tag at the C-terminus.
Key Features High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Time-saving: no need for transcription and translation. 
DNA-free: no external DNA added to system.

crRNA -dependent double-stranded DNA cleavage.

Source Recombinant Cas12a with an C-terminal NLS expressed by E.coli.
Species Acidaminococcus sp. (strain BV3L6)
Tag C-terminal 6× His Tag
Apparent Molecular Weight ~150 kDa, on SDS-PAGE under reducing conditions.
Concentration 4(±10%) mg/ml
Active temperature This Cas12a is active at 37℃.
Storage Buffer 20 mM Sodium Acetate, 500 mM NaCl, 1 mM TCEP,0.1 mM EDTA, 50% glycerol, PH6.0.
Storage & Stability This product remains stable up to 18 months at -20. Avoid repeated freeze-thaw cycles.
Accession# U2UMQ6

Appearance Clear, colorless liquid
Purity ≥ 90% by SDS-PAGE
Concentration 4(±10%) mg/m
Bioactivity ≥ 90%
Residual DNase Undetectable
Residual RNase Undetectable
Endotoxin Level ≤ 0.1 EU/μg of the protein by gel clotting method

  • GenCRISPR? Cas12a (Cpf1) Nuclease
  • GenCRISPR? Cas12a (Cpf1) Nuclease

    A 20 μl reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng GenCRISPR? Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.


References 1.    Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system." Cell 163.3 (2015): 759-771.
2.    Ledford, Heidi. "Alternative CRISPR system could improve genome editing." Nature News 526.7571 (2015): 17. 
3.    “Cpf1 Takes CRISPR Bigger by GoingSmaller.”

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


喜歡新升級的網站嗎?

討厭

不喜歡

一般

喜歡

非常喜歡

*
    主站蜘蛛池模板: 应用必备| 九台市| 织金县| 金堂县| 金沙县| 临洮县| 华阴市| 五大连池市| 甘南县| 汝城县| 秦安县| 玉门市| 开平市| 沧源| 肥乡县| 抚远县| 页游| 富源县| 海安县| 木兰县| 石狮市| 慈利县| 兴文县| 丰台区| 娱乐| 沙田区| 岢岚县| 原平市| 藁城市| 大关县| 景德镇市| 美姑县| 偃师市| 南陵县| 鄂托克前旗| 肥东县| 介休市| 灵丘县| 棋牌| 巧家县| 安义县|