| Species |
Human |
| Protein Construction |
IL-2 (Ala21-Thr153)
Accession # P60568 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
15.3 kDa |
| N-terminal Sequence Analysis |
The sequence of the first five N-terminal amino acids wa determined and found to be Met-Ala-Pro-Thr-Ser-Ser. |
| Apparent Molecular Weight |
15,300 Da |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H?O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 μg of IL-2, Human?, non-reducing (NR)
Lane 2: 2 μg of IL-2, Human?, reducing (R) )
> 95% as analyzed by SDS-PAGE
| Target Background |
Interleukin-2 (IL-2) is a Oglycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. |
| Synonyms |
IL2; IL-2; TCGF; lymphokine; interleukin 2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
