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| M00603 | |
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| Product Description | Recombinant CHO - K1 cells stably overexpress human FcRn and B2M subunit on the surface. The B2M subunits enable stable expression of FcRn in cells. Association of FcRn alpha - chain with beta (2) m is also important for IgG binding. The surface expression of FcRn is validated by FACS analysis. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 200 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies). |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. A5669701, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma). |
Figure 1. FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells. The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were probed using FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz Biotechnology) and PE conjugated Goat anti-mouse IgG (minimal x-reactivity) antibody (Cat. No. 405307, Biolegend). ?
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
