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| RC00010 | |
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| Description | ABE8e is an innovative adenine base editor designed to enable precise genetic modifications by converting A-T base pairs to G-C base pairs without introducing double-stranded breaks or requiring donor DNA templates.? ABE8e contains eight additional mutations in the TadA domain, augments the effectiveness and applicability of adenine base editing. |
| single guide RNA (sgRNA) dependent single-stranded DNA cleavage |
| References | David R Liu,et al.” Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity” Nat Biotechnol. 2020 Jul;38(7):883-891. |
| Purity | ≥90% by SDS-PAGE, 49% by SEC-HPLC |
| Residual DNase | Non-specific DNase activity |
| Endotoxin Level | Control <10EU/mg |
| Expression System | Recombinant Cas9-based fusion with double-ended NLS expressed by E.coli |
| Species | S. pyogenes |
| Tag | N-His tag |
| Molecular Weight | 185.140 Kda |
| Storage & Stability | ABE8e is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C. |
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