Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using
THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704)
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
A: 0.2 μg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 μg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 μg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
A: 0.2 μg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 μg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 μg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
20 μg Multiple Tag Cell Lysate (M0100) was used for the IP using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704) and half of th IP was loaded on the gel.
The Western blot was performed by One-Step IP-Western Kit (Mouse) (L00232)
Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.
Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 μg/ml) in assay.
The signal was developed with IRDye? 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
THE? c-Myc Tag Antibody, mAb, Mouse
| A00704 | |
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| ￥1500 | |
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