資源 » 參考數(shù)據(jù)庫 » 客戶發(fā)表文獻
| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | The open reading frames of?DES1?and?DES2?were codon optimised for expression in?S. cerevisiae?by Genscript, synthesised and supplied in the pUC-57 vector.?DES1?and?DES2?were then excised using?BamHI?and?SalI?restriction sites and ligated into pHEY1 [22], for expression under the constitutive?TEF1?promoter. pHEY-DES1, pHEY-DES2 and pHEY-EVC (empty vector) were transformed into?S. cerevisiae?[23] wild type strain DTY-11a (MATa, leu2-3, leu2-12, trp1–1, can1–100, ura3–1, ade2–1) and?ole1Δ?knockout strain AMY-3α (MATα, ole1Δ::LEU2, trp1–1, can1–100, ura3–1, ade2–1) [24] and colonies were selected on synthetic Dexterose (SD) minimal medium agar plates lacking uracil. | Get A Quote |
Arbuscular mycorrhizal fungi are oleaginous organisms and the most abundant fatty acyl moiety usually found in their lipids is palmitvaccenic acid (16:1Δ11cis). However, it is not known how this uncommon fatty acid species is made. Here we have cloned two homologs of Lepidopteran fatty acyl-CoenzymeA Δ11 desaturases from?Rhizophagus irregularis. Both?DES1?and?DES2?are expressed in intraradicle mycelium and can complement the unsaturated fatty acid-requiring auxotrophic growth phenotype of the?Saccharomyces cerevisiae ole1Δ?mutant. DES1 expression leads almost exclusively to oleic acid (18:1Δ9cis) production, whereas DES2 expression results in the production of 16:1Δ11cis?and vaccenic acid (18:1Δ11... More
