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目錄產(chǎn)品 » 穩(wěn)定細(xì)胞系 » Human Recombinant Growth Hormone Secretagogue Receptor Stable Cell Line

Figure 1: Ibutamoren -induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GHSR cells. The cells were loaded with Calcium-4 prior to being stimulated with a GHSR receptor agonist, Ibutamoren. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Ibutamoren (Mean ± SD, n = 2). The EC₅₀ of Ibutamoren on this cell was 14.28 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/GHSR Stable Cell Line

Ghrelin, a 28-amino-acid gut peptide is secreted from the stomach. It stimulates the release of growth hormone (GH) from the pituitary through GHS-R and plays important roles in energy homeostasis. In addition, ghrelin acts directly on the central nervous system to decrease sympathetic nerve activity. Ghrelin receptors (GHSRs) are concentrated in the hypothalamus-pituitary unit and may take the role of ghrelin in memory. GHSR is distributed in peripheral tissues, including the heart, lung, liver, kidney, pancreas, stomach, small and large intestines, adipose, and immune cells, suggesting that ghrelin has multiple functions in these tissues.

產(chǎn)品編號(hào)	M00189
規(guī)格	2 vials 大單詢價(jià)
數(shù)量
價(jià)格	詢價(jià)


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Synonyms

ghrelin receptor, GHSR-1a, growth hormone secretagogue receptor, GHS-R1a (Growth hormone secretagogue receptor type 1), growth hormone-releasing peptide receptor, GH-releasing peptide receptor, GHRP, GHS-R, C530020I22Rik, ghrelin receptor 1a

產(chǎn)品概述

Applications

Functional assay for GHSR receptor

產(chǎn)品簡介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培養(yǎng)條件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 500 μg/ml G418 (Cat. #10131-035, Gibco)