Figure 1: ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRB receptor agonist ET-1. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ET-1 (Mean ± SD, n = 2). The EC₅₀ of ET-1 on EDNRB expressing CHO-K1 cells was 41.5 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/EDNRB Stable Cell Line

In mammals, the endothelin (ET) family comprises three endogenous isoforms: ET-1, ET-2, and ET-3. These endothelium-derived peptides perform their functions via two endothelin receptors, classified as EDNRA and EDNRB. EDNRB receptors present on the endothelial cells lining the vessel wall and play a role in the release of endothelium-derived relaxing factors such as nitric oxide (NO) and prostanoids and in removing ET from the circulation.

產(chǎn)品編號(hào)	M00150
規(guī)格	2 vials 大單詢價(jià)
數(shù)量
價(jià)格	詢價(jià)


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Synonyms

ETB receptor, HSCR2, HSCR, ETB, Ednra, ET-B, ET-BR, endothelin B receptor, endothelin receptor, endothelin receptor non-selective type, endothelin receptor type B, endothelin-B receptor, Sox10m1, ETR-b, AU022549

產(chǎn)品概述

Applications

Functional assay for EDNRB receptor

產(chǎn)品簡介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培養(yǎng)條件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)

圖例

Figure 1: ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRB receptor agonist ET-1. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ET-1 (Mean ± SD, n = 2). The EC₅₀ of ET-1 on EDNRB expressing CHO-K1 cells was 41.5 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

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CHO-K1/EDNRB Stable Cell Line

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