| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | The sequence for batVP35 was synthesized (Genscript, Piscataway, NJ (codon optimized for E. coli expression)) based on a previously described Myotis lucifigus VP35 sequence and cloned with an amino-terminal Flag-tag into pCAGGS (Belyi et al., 2010).Sequences were synthesized for Myotis davidii VP35 (GenBank: ALWT01033109.1, nucleotides 1842-2562) and Myotis brandtii VP35 (GenBank: ANKR01158691.1, nucleotides 2885-3727) and were similarly tagged and cloned (Genscript, Piscataway, NJ).The resulting sequence was synthesized and cloned as above (Genscript, Piscataway, NJ). | Get A Quote |
Non-retroviral integrated RNA viral sequences (NIRVs) potentially encoding ～280 amino acid homologs to filovirus VP35 proteins are present across the Myotis genus of bats. These are estimated to have been maintained for ～18 million years， indicating their co-option. To address the reasons for co-option， 16 Myotis VP35s were characterized in comparison to VP35s from the extant filoviruses Ebola virus and Marburg virus， in which VP35s play critical roles in immune evasion and RNA synthesis. The Myotis VP35s demonstrated a conserved suppression of innate immune signaling， albeit with reduced potency， in either human or Myotis cells. Their attenuation reflects a lack of dsRNA binding that in the filov... More
