PreScission Protease

PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro (LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.

價格	￥800
產品編號	Z02799
規格	100 IU 250 IU 5000 IU 大單詢價
數量

產品概述產品屬性應用指導圖例

Description

Synonyms

PreScission Protease(PSP);

Note

This material is offered for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Not available in the US or Canada.

產品概述

Source

E. coli

產品屬性

反應緩沖液	Cleavage Buffer: 50mM Tris-HCl, pH7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5°C prior to use.
Physical Appearance	Sterile colorless liquid.
Biological Activity	Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 90% completion at 5°C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. Cleavage Buffer: 50 mM Tris-HCl, pH 7.0 (at 25 °C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use.
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE
Storage & Stability	Upon receiving, this product remains stable for up to 6 months at -20 °C or below. Avoid repeated freeze-thaw cycles.

應用指導

During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding Triton^TM X-100, Tween^TM 20, Nonidet^TM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.

圖例

Lane 1: 2 μg of PreScission Protease, non-reducing (NR)
Lane 2: 2 μg of PreScission Protease, reducing (R)
≥ 90% as analyzed by SDS-PAGE ?

相關文獻

1. Shantel Rios, et al. The Development of a Rabies Virus-Vectored Vaccine against , Targeting BBI39. Vaccines (Basel). (2024-01)
2. Mitchell A Ellison, et al. Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiae. Nucleic Acids Res. (2023-06)
3. Asrafun Nahar, et al. Assembly checkpoint of the proteasome regulatory particle is activated by coordinated actions of proteasomal ATPase chaperones. Cell Reports. (2022-06)
4. Fatma Betul Ertem, et al. Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography. STAR Protoc. (2022-01)
5. Fatma Betul Ertem, et al. Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography. STAR Protoc. (2022-01)
6. English JG, et al. VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells. Cell. (2019-07)

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PreScission Protease

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