| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | by sodium dodecyl Proteins were concentrated using Amicon? filters with a 3 kDa cutoff, resuspended in 50 mM Tris (pH 7), and tested for endotoxin content by the Limulus amebocyte lysate assay using the ToxinSensor Gel clot endotoxin assay kit (Genscript, New Jersey) according to the manufacturer's instructions. | Get A Quote |
The Burkholderia cepacia complex (Bcc) is a group of Gram-negative opportunistic pathogens causing infections in people with cystic fibrosis (CF). Bcc is highly antibiotic resistant, making conventional antibiotic treatment problematic. The identification of novel targets for anti-virulence therapies should improve therapeutic options for infected CF patients. We previously identified that the peptidoglycan-associated lipoprotein (Pal) was immunogenic in Bcc infected CF patients; however, its role in Bcc pathogenesis is unknown. The virulence of a pal deletion mutant (Δpal) in Galleria mellonella was 88-fold reduced (p?More
