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Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay.

Biomark Insights. 2017;

Radhakrishnan J, Origenes R, Littlejohn G, Nikolich S, Choi E, Smite S, Lamoureux L, Baetiong A, Shah M, Gazmuri RJ,.

Products/Services Used	Details	Operation
Catalog Antibody	Capture antibody and horseradish peroxidase (HRP) labelled detection antibody, both specific for rat cytochrome c and rat cytochrome c standard (Cat. No. DYC897, DuoSet IC, Rat/Mouse Total Cytochrome c kit), were from R&D systems (McKinley Place NE, MN, USA). Anti-HRP antibody (Cat. No. A00619) was obtained from GenScript (Piscataway, NJ, USA).	Get A Quote

摘要

Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury.,We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200?ng/mL in humans and 5-500?ng/mL in rat) and high assay reproducibility (inter-assay coefficient <6% in humans and <10% in rat). In patients after blunt trauma, plasma cytochrome c directly correlated with injury severity. In rats after cardiac resuscitation, plasma cytochrome c inversely correlated with survival and responsiveness to mitochondrial protective interventions.,The cytochrome c assays herein presented have high s... More