| Products/Services Used | Details | Operation |
|---|---|---|
| Polyclonal Antibody Services | To assay for LecB binding to Psl, Protein G Dynabeads (Life Technologies, Carlsbad, CA) were incubated with 100?μg/mL anti-LecB polyclonal antibodies (GenScript, Piscataway, NJ) following the manufacturer’s instructions, and then incubated with a mixture of purified Psl and LecB or with only purified LecB (Sigma-Aldrich, St. Louis, MO) for 1?h at 37?°C on a low-speed rotator. | Get A Quote |
Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS)， exogenous DNA， and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB， which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here， we demonstrate that LecB binds to Psl， a key matrix EPS， and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally， we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix prote... More
