| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … Protein samples were subjected to SDS-PAGE, in-gel digestion and MS/MS analysis as described previously [6] 23 Purification of recombinant AP-57 and antibody generation AP-57 nucleic acid sequence were synthesized by GenScript Corporation (Nanjing, China) … | Get A Quote |
The effectiveness of a drug may be highly dependent on its delivery to its target organ and even to specific intracellular organelles. In this study we developed nanoparticles (NPs) composed of the anionic polypeptide poly-γ-glutamic acid (γ-PGA)， and a designed amphiphilic and cationic β-sheet peptide (PFK)， which tends to form fibril bilayer assemblies. These peptide assemblies generate hydrophobic niches within the NPs， which enhance the NPs' capacity to deliver amphiphilic drugs. NPs created by coassembly of γ-PGA and PFK， and further coated with PFK， had a positive zeta-potential and were attracted to mitochondria. When applied to the human osteosarcoma cell line Saos2， the NP-encapsulated ... More
