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Small RNA zippers lock miRNA molecules and block miRNA function in mammalian cells

Biology of the Cell. 2016;

Heidi Gytz, Mariann F. Hansen, Signe Skovbjerg, Anders C. M. Kristensen, Sofie H?rlyck, Mette B. Jensen, Marlene Fredborg, Lotte D. Markert, Nigel A. McMillan, Erik I. Christensenand Pia M. Martensen

Products/Services Used	Details	Operation
Recombinant Proteins	polyacrylamide gel electrophoresis (SDS-PAGE) or 17% Tricine SDS-PAGE (Martensen et al., 2001). Proteins were then transferred to poly(vinylidene difluoride) (PVDF) membranes (Millipore) by electroblotting. Immunoblot analysis was carried out using antibodies raised against PARP (Cell signalling, 1:2.000), HSV (Novagen, 1:10.000), FLAG (M2 from Sigma, 1:4.000 or THE-FLAG antibody from Genscript, 1:1.000), Bcl-2 (Sigma, 1:5.000), ANT (Santa Cruz Biotechnology, 1:550), VDAC1 (Abcam, 1:200), or Mcl-1 (Santa Cruz Biotechnology, 1:1.000)	Get A Quote

摘要

Background information: Interferons are a family of cytokines with growth inhibitory and antiviral functions, which exert their biological actions through the expression of Interferon Stimulated Genes, ISGs. The human ISG12 family of proteins comprises ISG12A, ISG12B, ISG12C and ISG6-16. Due to differential splicing and a gene variation, the human ISG12A protein exists as a full-length ISG12A form and three ISG12A variants. ISG12 has been found transcriptionally dysregulated in many disorders. High levels of ISG12A mRNA have been found in breast and ovarian cancers. Loss of heterozygosity at the position of the ISG12 genes often occurs in ovarian carcinomas and lymphoblastic leukemias. Both ISG12A and ISG6-16 a... More