Reconstructed ancestral enzymes suggest long-term cooling of Earth's photic zone since the Archean
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coli BL21 cells. The ORF42 protein was expressed under 0.1 mM isopropyl β-D1-thiogalactopyranoside (IPTG) induction at 16?C overnight, purified from the soluble fraction using a Ni-nitrilotriacetic acid (NTA) column (Genscript, Nanjing, China) according to the manufacturer’s instructions, and then analyzed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Gu et al., 2014) |
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Identification and Characterization of Dpo42, a Novel Depolymerase Derived from the Escherichia coli Phage vB_EcoM_ECOO78.
Identification and Characterization of Dpo42, a Novel Depolymerase Derived from the Escherichia coli Phage vB_EcoM_ECOO78.
: bacteriophage, depolymerase, Escherichia coli, biofilm, beta-lactamase
