Rabies vaccine administration is the most effective method to prevent the occurrence of rabies disease. However， administration of rabies vaccine without adjuvant always shows low efficiency. As a member of superantigen， staphylococcal enterotoxin C2 (SEC2) non-specifically activates T-cells at extremely low concentration. It enlightens us that SEC2 may be used as an adjuvant. We carried out the experiment that the mice received twice immunization with rabies vaccine in the presence or absence of SEC2 at 1-week interval. Serum and splenocytes from immunized mice were collected to measure the level of rabies-specific-IgG and the cell that secretes IFN-γ or IL-4. The promotion of antigen-specific splenocytes proliferation was also detected. Besides， a challenge test was performed to evaluate the protective efficiency of SEC2. It was shown that mice immunized with vaccine combined with SEC2 generated more specific anti-rabies-antibodies. The results for production of IFN-γ and IL-4， as well as the proliferation of splenocytes from immunized mice indicated SEC2 promoted the specific immune responses induced by rabies vaccine. Moreover， immunization of mice with vaccine combined with SEC2 provided efficient protection against the lethal rabies exposure. Taken together， our findings indicated that SEC2 can be served as an adjuvant for rabies vaccines.