| ￥1050 | |
| RC00003-30 | |
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| Description | PEmax RNase deletion involves modifications to the PEmax enzyme aimed at eliminating unwanted RNase activity, thus enhancing its stability and specificity. This advancement allows for more efficient target editing by minimizing the degradation of RNA components involved in the editing process, further improving the overall performance of the prime editing protocol. |
| single guide RNA (sgRNA) dependent single-stranded DNA cleavage |
| References | David R Liu,et al.“Phage-assisted evolution and protein engineering yield compact, efficient prime editors”. Cell. 2023 Aug 31;186(18):3983-4002.e26. |
| Purity | ≥90% by SDS-PAGE, 98% by SEC-HPLC |
| Residual DNase | Non-specific DNase activity |
| Endotoxin Level | Control <10EU/mg |
| Expression System | Recombinant Cas9-based fusion with three NLS expressed by E.coli |
| Species | S. pyogenes |
| Tag | C-His tag |
| Molecular Weight | 223.714KDa |
| Storage & Stability | This product is supplied with storage buffer (50 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.5, at 25°C) and recommended to be stored at -80°C. |
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