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Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes.

J Biol Methods. 2018; 
Morran MP, Al-Dieri AG, Nestor-Kalinoski AL, Jordan RK, Gupta NK, McInerney MF,.
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Catalog Antibody DYKDDDDK FLAG resin immunoprecipitation Protein extracts from the IR stimulation assay were subjected to immunoprecipitation of DYKDDDDK FLAG-tagged protein via an anti-DYKDDDDK G1 affinity resin (GenScript L00432-1) to separate native IR from the 3×FLAG-mIR. Get A Quote

摘要

The insulin receptor (IR) is a transmembrane receptor which recognizes and binds the hormone insulin. We describe two models that were devised to explore the role of IR over-expression on T-lymphocytes and their chemotactic motility in the progression of type 1 diabetes. FVB/NJ-CD3-3×FLAG-mIR/MFM mice were generated to selectively over-express 3×FLAG tagged murine IR in T-lymphocytes via an engineered CD3 enhancer and promoter construct. Insertion of the 3×FLAG-mIR transgene into FVB/NJ mice, a known non-autoimmune prone strain, lead to a minor population of detectable 3×FLAG-mIR tagged T-lymphocytes in peripheral blood and the presence of a few lymphocytes in the pancreas of the Tg+/- compared to age match... More

關(guān)鍵詞

Cre-recombinase; chemotaxis; diabetes; insulin receptor; insulitis
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