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A fast and easy one-step purification strategy for plant-made antibodies using Protein A magnetic beads

Front Plant Sci. 2024-01;

Lo?c Faye, Clemens Grünwald-Gruber, Louis-Philippe Vezina, Véronique Gomord, Bertrand Morel

Products/Services Used	Details	Operation
Catalog Antibody	… for immunodetection at a concentration of 0.36 μg/mL followed by a secondary mouse anti-human IgG Fc antibody coupled to horseradish peroxidase at a 1:1,000 dilution (Genscript, …	Get A Quote

摘要

A major difficulty to reach commercial- scale production for plant-made antibodies is the complexity and cost of their purification from plant extracts. Here, using Protein A magnetic beads, two monoclonal antibodies are purified in a one-step procedure directly from non-clarified crude plant extracts. This technique provides significant savings in terms of resources, operation time, and equipment.