| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Goat anti-rabbit IgG monoclonal antibody [HRP] and goat anti-rabbit IgG monoclonal antibody were purchased from GenScript Bio.Co.Ltd (Nanjing, China). Vancomycin monoclonal antibody [immunogen was Van-BTG (bovine thyroglobulin, BTG)] was purchased from United States Biological Company (USA). Whole milk powder, skim milk powder and instant milk powder were purchased from Suguo supermarket (Nestle, Switzerland). Other reagents were all purchased from GE Healthcare (Beijing, China). | Get A Quote |
Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive time-resolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong cross-reactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stab... More
