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Synergistic Effect of Polysaccharide Gums and Antimicrobial Agents on Susceptibility and Protein Expression of Select Pathogenic Microorganisms in Milk

Journal of Food Research. 2018;

Bernice Karlton-Senaye , Sarah Adjei-Fremah , Mulumebet Worku & Leonard Williams

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Recombinant Proteins	Endotoxin assay was done on all solution and diluents used in this study following the procedure used by Adjei-Fremah et al. (2016). The ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit (GenScript, Piscataway, NJ) was used following the manufacturer’s protocol.	Get A Quote

摘要

The quest for the use of natural ingredients as preservatives and antimicrobial agents is rising. Polysaccharide gums are usually used as emulsifying agents and as preservatives. The objective of this study was to investigate the combined effect of five different polysaccharide gums and antimicrobial agents on growth, susceptibility and protein expression of select pathogenic microorganisms in milk. Antimicrobial susceptibility and protein concentration were determined by disc diffusion and Pierce BCA assay, respectively. The proteome pattern and the number of protein spots were determined by two-dimensional gel electrophoresis. The results showed that xanthan (6.68±0.02 Log CFU/mL) caused the most growth inhi... More