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Enhanced Population Control in a Synthetic Bacterial Consortium by Interconnected Carbon Cross-Feeding.

ACS Synth Biol. 2019-12; 
Losoi PS, Santala VP, Santala SM.
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摘要

Engineered microbial consortia can provide several advantages over monocultures in terms of utilization of mixed substrates, resistance to perturbations, and division of labor in complex tasks. However, maintaining stability, reproducibility, and control over population levels in variable conditions can be challenging in multispecies cultures. In our study, we modeled and constructed a synthetic symbiotic consortium with a genetically encoded carbon cross-feeding system. The system is based on strains of Escherichia coli and Acinetobacter baylyi ADP1, both engineered to be incapable of growing on glucose on their own. In a culture supplemented with glucose as the sole carbon source, growth of the two strains is... More

關鍵詞

consortium modeling; cross-feeding; population control; synthetic consortium
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