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Activation of Wnt Signaling Increases Numbers of Enteric Neurons Derived From Neonatal Mouse and Human Progenitor?Cells

Gastroenterology. 2017-07;

ZhangYing, SeidKarin, ObermayrFlorian, JustLothar, NeckelPet

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Recombinant Proteins	… Ten micrograms of each protein sample were loaded onto a 12.5% (w/v) sodium dodecyl sulfate polyacrylamide gel and blotted onto a nitrocellulose membrane. Detection was carried out with the ONE-HOUR Western Standard Kit (GenScript, Piscataway, NJ) …	Get A Quote

摘要

Neural stem and progenitor cells from the enteric nervous system (ENS) might serve as a source of cells for treatment of neurogastrointestinal disorders. Before we can use these cells， we must increase our understanding of the signaling mechanisms that regulate proliferation and differentiation. We systematically evaluated the effects of canonical Wnt signaling on proliferation and differentiation of cultured ENS progenitor cells from neonatal mice and humans.