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Comparison of enzyme immunoassays for detection of antibodies to hepatitis D virus in serum.

Clin Vaccine Immunol.. 2016-08;

Chow SK, Atienza EE, Cook L, Prince H, Slev P, Lapé-Nixon M, Jerome KR.

Products/Services Used	Details	Operation
Catalog Products	... assay. Briefly, serum samples diluted 1:101 in phosphate buffered saline with 0.1% 60 tween 20 (PBST) containing 0.1% bovine serum albumin were added to microtiter wells 61 coated with a proprietary recombinant HDV protein (GenScript, Piscataway,NJ). After 62 ...	Get A Quote

摘要

Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection.