Immobilized IL-2,Human (Cat.No.Z03074) at 5μg/mL (100 μL/well) can bind CD25/IL-2Rα, Fc Chimera,Human with a linear range of 1.2-11 ng/mL.

CD25/IL-2Rα Fc Chimera, Human

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rβ, and IL-2Rγ. The IL-2Rα is a type I transmembrane protein consisting of a 219 amino acid (a.a.) extracellular domain, a 19 a.a. transmembrane domain and a 13 a.a. intracellular domain, which is not involved in the transduction of IL-2 signal. Activated T cells, regulatory T cells (Tregs) and NK cells express high levels of CD25 and expression of the high-affinity IL-2Rα is mostly limited to these cell populations. Signaling via IL-2Rα mediates multiple biological processes in various cell populations, e.g. proliferation and differentiation of B cells and NK cells. A soluble form of IL-2Rα (IL-2Rα) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2Rα is unclear. Increased levels of IL-2Rα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2Rα has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

產品編號	Z03400
規格	10 μg 50 μg 大單詢價
數量
價格	￥1200


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產品簡介

Species

Human

Protein Construction

CD25/IL-2Rα (Glu22-Cys213) Accession # P01589	hFc
N-term	C-term

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

Immobilized Human IL-2 at 5.0 μg/ml (100 μl/well) can bind CD25/IL-2Rα, hFc, Human.

Expression System

HEK 293

Apparent Molecular Weight

65~75 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

Immobilized IL-2,Human (Cat.No.Z03074) at 5μg/mL (100 μL/well) can bind CD25/IL-2Rα, Fc Chimera,Human with a linear range of 1.2-11 ng/mL.

技術背景

Target Background

The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rβ, and IL-2Rγ. The IL-2Rα is a type I transmembrane protein consisting of a 219 amino acid (a.a.) extracellular domain, a 19 a.a. transmembrane domain and a 13 a.a. intracellular domain, which is not involved in the transduction of IL-2 signal. Activated T cells, regulatory T cells (Tregs) and NK cells express high levels of CD25 and expression of the high-affinity IL-2Rα is mostly limited to these cell populations. Signaling via IL-2Rα mediates multiple biological processes in various cell populations, e.g. proliferation and differentiation of B cells and NK cells. A soluble form of IL-2Rα (IL-2Rα) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2Rα is unclear. Increased levels of IL-2Rα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2Rα has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Synonyms

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2Rα

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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CD25/IL-2Rα Fc Chimera, Human

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