M-CSF, Mouse?

Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain, and it is only active in the disulfide-linked dimeric form, which is bonded at Cys63.

價格	￥600
產品編號	Z03275
規格	10 μg 50 μg 大單詢價
數量

產品簡介圖例技術背景

產品簡介

Species

Mouse

Protein Construction

Expressed with an N-terminal Met.

M-CSF (Lys33-Pro187)
Accession # P07141

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 3.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 3.3 × 10⁵ units/mg.

Expression System

E. coli

Apparent Molecular Weight

~30 kDa, on SDS-PAGE under non-reducing conditions.

Formulation

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

圖例

ED₅₀ < 3.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 3.3 × 10⁵ units/mg. ?

Lane 1: 2 μg of M-CSF, Mouse , non-reducing (NR)
Lane 2: 2 μg of M-CSF, Mouse , reducing (R)
> 95% as analyzed by SDS-PAGE ?

技術背景

Target Background

Synonyms

CSF1; CSF-1; MCSF; colony stimulating factor 1; Macrophage Colony Stimulating Factor; Lanimostim; MGC31930; MGI-IM

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相關文獻

1. Oyler-Yaniv J, et al. Catch and Release of Cytokines Mediated by Tumor Phosphatidylserine Converts Transient Exposure into Long-Lived Inflammation. Mol Cell. (2017)

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M-CSF, Mouse?

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