| Species |
Human |
| Protein Construction |
BD-3 (Gly23-Lys67)
Accession # P81534 |
|
| Purity |
> 98% as analyzed by SDS-PAGE
> 98% as analyzed by HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli is less than 30.0 μg/ml, corresponding to a specific activity of > 33.3 IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
5.2 kDa |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 20 mM PBS, pH 7.4, 130 mM NaCl. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. |
| Synonyms |
Beta-defensin 103; Beta-defensin 3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein; DEFB103A; BD3; DEFB103; DEFB3 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
