HEK293/Luc (IL-6) Stable Cell Line
HEK293/Luc (IL-6) Stable Cell Line is designed for cell-based functional assays that measure the bioactivity of the drug targeting IL-6.
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| M00954 | |
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| 詢價 | |
| M00954 | |
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| Product Description |
Recombinant
HEK293 cells stably overexpress SIE (sis-inducible element)-Luc on the surface and
activate the receptor to the downstream signaling pathways upon the binding of IL-6.
The surface expression of SIE-Luc is validated by luminescence analysis. This
stable cell line product is designed for cell-based functional assays that
measure the bioactivity of the drug targeting IL-6. |
| Culture Properties |
Adherent |
| Stability |
Stable through more than 15 passages with no
significant changes in assay performance or expression profile. |
| Size |
2 vials of frozen cells (>1X106 per vial
in 1 ml) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium |
DMEM, 10% FBS, 250 μg/ml Geneticin (Cat. No. 10131-035,
Gibco) |
| Complete Growth Medium |
DMEM, 10% FBS |
| Freeze Medium-DATA |
42% DMEM (Cat. No.
10569-010, Gibco), 50% FBS (Cat. No.10099-141, Gibco), 8% DMSO (Cat. No. D2650,
Sigma) |
Figure 1. Luminescence analysis of cell surface expression of SIE-Luc on HEK293 cells. The HEK293/Luc (IL-6) cells were stimulated by IL-6. Fire-Lumi? Reagent (Cat. No. L00877C-1000, Genscript) was used and luminescence measured by PHERAstar FSX.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.
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