Figure 1. FACS analysis of cell surface expression of CD32A 131Arg on CHO-K1/CD32A 131Arg cells. The CHO-K1/CD32A 131Arg cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).

CHO-K1/CD32A 131Arg Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa/CD32A 131Arg) on the surface. The surface expression of CD32A 131Arg is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131Arg. GenScript also offers CD32A 131His stable cell line (Cat. No. M00598) for FcγRIIa polymorphism study.

產品編號	M00887
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human immunoglobulin gamma Fc region receptor II-a (FcγRIIa/CD32A 131Arg) on the surface. The surface expression of CD32A 131Arg is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32A 131Arg. GenScript also offers CD32A 131His stable cell line (Cat. No. M00598) for FcγRIIa polymorphism study.
Culture Properties	Adherent.
Stability	Stability: Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

圖例

Figure 1. FACS analysis of cell surface expression of CD32A 131Arg on CHO-K1/CD32A 131Arg cells. The CHO-K1/CD32A 131Arg cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated Mouse Anti-Human CD32 antibody (Cat. No. 559769, BD Bioscience).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/CD32A 131Arg Stable Cell Line

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