Figure 1. FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells. The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated mouse anti-human CD16 (Cat. No. 302008, Biolegend).

CHO-K1/CD16B NA1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb/CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development.

產品編號	M00602
規格	2 vials 大單詢價
數量
價格	詢價


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產品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb/CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development.
Culture Properties	Adherent
Stability	Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1. FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells. The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated mouse anti-human CD16 (Cat. No. 302008, Biolegend).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/CD16B NA1 Stable Cell Line

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