Figure 1: FACS analysis of cell surface expression of PD-L1 on CHO-K1 cells. The CHO-K1/PD-L1 cells (red) and the negative control CHO-K1 cells (green) were probed using Anti-Human PD-L1 (GenScript) and Alexa Fluor? 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

CHO-K1/PD-L1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface expression of PD-L1 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies.

產(chǎn)品編號	M00543
規(guī)格	2 vials 大單詢價
數(shù)量
價格	詢價


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產(chǎn)品簡介

Product Description	Recombinant CHO-K1 cells stably overexpress human programmed cell death 1 ligand 1 (PD-L1) on the surface. The surface expression of PD-L1 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies.
Culture Properties	Adherent
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培養(yǎng)條件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

圖例

Figure 1: FACS analysis of cell surface expression of PD-L1 on CHO-K1 cells. The CHO-K1/PD-L1 cells (red) and the negative control CHO-K1 cells (green) were probed using Anti-Human PD-L1 (GenScript) and Alexa Fluor? 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/PD-L1 Stable Cell Line

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